LDL; HOCL OX; RBX HU-100UL

Application

This Anti-Low Density Lipoprotein Antibody, HOCL oxidized is validated for use in ELISA, IH for the detection of Low Density Lipoprotein.

Research CategoryMetabolism

Research Sub CategoryLipid Metabolism & Weight Regulation

Immunohistochemistry: 1:50-1:400

ELISA: 1:400-1:4,000

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Hypochlorite-modified LDL.

Epitope: HOCL oxidized

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit serum.

Specificity

Reacts strongly and very specifically with HOCL-LDL. By time resolved fluorescence there was no detectable cross reactivity to unmodified LDL has been observed. However weak cross reactivity with MDA-LDL (20%), HNE-LDL (5%) and minimally modified LDL (5%) was observed. By Western blot no detectable cross reactivity to unmodified LDL was observed. Weak cross reactivity (﹤10%) was detected with MDA-LDL, HNE-LDL and 24 hour Cu2+ oxidized LDL. By ELISA the antibody bound to early and late stages of HOCL-LDL but only weakly (﹤5%) to several other modifications including MDA-LDL, Cu2-ox-LDL, MDA-HSA and native LDL.

Storage and Stability

Maintain lyophilized material at -20°C for up to 6 months After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.